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Essential Duties and Responsibilities
- Protein production and purification using bacterial and insect expression systems. Characterization of protein-protein interactions in vitro
- Assembly of protein complexes and prepare vitrified samples for single-particle cryo-electron microscopy analysis
- Transient expression of GFP-tagged proteins in protoplasts or Tabacco leaves and perform subcellular localization experiments using live-cell imaging
- Use cryo-focused ion beam (cryo-FIB) to prepare thin samples for cryo-electron cellular tomography
- Collect single-particle and electron tomography data and perform data analysis
- Dissemination of the research results as progress reports as well as peer-reviewed publications
Required Knowledge, Skills & Abilities:
- Ph.D. in molecular biology, biochemistry, biophysics, chemistry, cell biology, plant biology, plant genetics, microbiology, immunology, virology, or a related field
- Demonstrated experience in cell culture and protein production through over-expression
- Proven abilities for dissemination of research through publication in peer-reviewed journals
- Ability to communicate clearly, effectively and professionally
- Self-motivated and able to work both independently and as part of a team
Preferred Knowledge, Skills & Abilities:
- Experience in protein production in a eukaryotic system such as insect, yeast, or mammalian cells
- Transient gene expression in plant leaves or protoplasts
- Experience in production and analysis of protein complexes or membrane proteins
- Experience in cryo-electron microscopy sample preparation, data collection and analysis
- Experience in cryo-electron tomography sample preparation, data collection, and analysis
- Experience in use of a cryo-focused ion beam (cryo-FIB) for milling biological samples
- Experience in studying protein-protein interactions using fluorescence, affinity purification, and Kd measurements
- Experience in biological crystallography and diffraction data collection and analysis
Other information:
- Review of applications begins immediately. Applications will be accepted until the position is filled
- Initial 1-year appointment subject to annual renewal contingent on performance and funding
- Research is under the direction of Dr. Qun Liu
- BNL policy requires that after obtaining their PhD, eligible candidates for research associate appointments may not exceed a combined total of 5 years of relevant work experience as a post-doc and/or in an R&D position, excluding time associated with family planning, military service, illness or other life-changing events
- This is a fully onsite position located at BNL
- Candidates should submit a CV, a brief description of research interests in a cover letter, and contact information for three references
At Brookhaven National Laboratory we believe that a comprehensive employee benefits program is an important and meaningful part of the compensation employees receive. Our benefits program includes, but is not limited to:
- Medical Plans
- Dental Plans
- Vacation
- Holidays
- Life Insurance
- 401(k) Plan
- Paid Parental Leave
- Swimming Pool, Weight Room, Tennis Courts, and many other employee perks and benefits
About Brookhaven National Laboratory
Brookhaven National Laboratory is a multipurpose research institution funded primarily by the U.S. Department of Energy’s Office of Science. Located on the center of Long Island, New York, Brookhaven Lab brings world-class facilities and expertise to the most exciting and important questions in basic and applied science—from the birth of our universe to the sustainable energy technology of tomorrow. We operate cutting-edge large-scale facilities for studies in physics, chemistry, biology, medicine, applied science, and a wide range of advanced technologies. The Laboratory's almost 3,000 scientists, engineers, and support staff are joined each year by more than 4,000 visiting researchers from around the world. Our award-winning history, including seven Nobel Prizes, stretches back to 1947, and we continue to unravel mysteries from the nanoscale to the cosmic scale, and everything in between. Brookhaven is operated and managed by Brookhaven Science Associates, which was founded by the Research Foundation for the State University of New York on behalf of Stony Brook University, and Battelle, a nonprofit applied science and technology organization.
https://careers.entsoc.org/jobs/18729760/res-assoc-biology
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